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Purified RNA was eluted into 50–100 μl using the supplied elution buffers, immediately frozen at or below −70 °C, and transported on dry ice.

The elution fractions containing protein with the highest molecular weight were further examined by negative-stain electron microscopy, and samples containing the holotoxin complex were finally used for plunge freezing and cryo-EM.

“This approval marks the start of Cook’s program to bring the benefits of drug elution to U.S. physicians treating the peripheral arteries,” said a Cook Medical executive, Rob Lyles.